Study on Allergenicity Assessment of hygromycin B phosphotransferase Protein 潮霉素磷酸转移酶的致敏性评价研究
Objective To investigate the allergenicity on HPT ( hygromycin B phosphotransferase) protein with BN rat model. 目的用BN大鼠致敏模型对HPT蛋白的致敏性进行研究。
The regenerated fertile plants were obtained by effective selection for hygromycin B resistance. 幼胚诱导的愈伤组织,经潮霉素抗性筛选,得到可育的再生植株。
Several lines showing resistance to hygromycin B were also analyzed with Southern blotting. The results further confirmed that ThEn-42 gene could stably inherit to T1 generation. 选择部分T1代潮霉素抗性植株进行Southern杂交鉴定,结果证实,外源基因已经稳定遗传给T1代。
Tagging of nematode-trapping fungus with hygromycin B phosphotransferase gene and its application in the study of fungistasis of soil 捕食线虫真菌的潮霉素抗性基因标记及其在土壤抑菌研究中的应用
The validity of target genes transferred by rice floral organ-mediated method were verified by PCR analysis, evaluation of resistance to sheath blight and blast, screening by hygromycin B and examination of GUS activity in transgenic rice. 经潮霉素(hygromycin)筛选、PCR分析、抗稻纹枯病和稻瘟病鉴定、GUS活性检测转基因水稻后代,验证了水稻花器介导法转目标基因的有效性。
Methods of hygromycin B phosphotransferase activity assay in transgenic plant 转基因作物标记蛋白潮霉素磷酸转移酶活性的测定方法
All of the transgenic rice with hygromycin phosphotransferase gene ( hpt) was resistant to hygromycin B, none appeared any toxic symptoms over the concentrations mentioned above. 在上述浓度范围内,带潮霉素磷酸转移酶基因(hpt)的Bt转基因水稻(克螟稻)幼苗均未出现中毒症状。
Study on transformation of P-dissolving Penicillium oxalicum P8 with double-marker vector expressing green fluorescent protein and hygromycin B resistance 绿色荧光蛋白和潮霉素抗性双标记载体转化草酸青霉菌P8的研究
Stable expression stains were screened by Hygromycin B and limited dilution method. 氨苄青霉素板筛选及PCR鉴定,挑出阳性克隆进行扩增、转染cos-7细胞并经潮霉素及有限稀释法筛选稳定表达克隆。
A transformation system based on a dominant selectable marker, hygromycin B resistance ( hyg B ′) gene, was established for Pyricularia oryzae. 利用抗药性标记基因(HygromycinB:hygB)建立稻瘟病菌的转化系统。
Objective: To produce monoclonal antibodies ( McAb) against hygromycin B phosphotransferase ( HPT) and to establish a rapid method for the measurement of HPT antigen in the genetically modified crops ( GMC). 目的:制备抗潮霉素B磷酸转移酶(HPT)的单克隆抗体(McAb),建立一种快速检测转基因作物中该选择标记基因HPT编码蛋白的方法。
Hygromycin B phosphotransferase ( HPT) is a widely used selectable marker protein of transgenic plant. 潮霉素磷酸转移酶(HPT)是转基因作物中重要的标记蛋白,其活性的测定对含有该标记基因的转基因作物的植株构建、基因沉默、标记基因去除及安全性分析等具有重要的作用。
The PCR analysis and hygromycin B resistant analysis of some T2 plants also demonstrated that ThEn-42 gene had stably inherited to T2 generation. 2. 对部分T2代株系进行潮霉素抗性鉴定和PCR鉴定,结果表明ThEn-42已经稳定遗传给T2代植株。
And the transformants resistance ability to hygromycin B increased greatly than the original strain. 且标记后菌株较之原始菌株的抗潮霉素能力大大增加。
Transformants which could express hygromycin B resistance genes effectively and steadily were obtained. The transformants maintained resistance to hygromycin B even after subculturing for 9 times. 筛选出稳定表达潮霉素B抗性的转化菌株,继代培养9代后仍有潮霉素抗性。